Zerumbone Attenuates the Severity of Acute Necrotizing Pancreatitis and Pancreatitis-Induced Hepatic Injury

Table 2

Experimental design of the formal study.

Group	Time
	−0.5h	0h	1h	3h	6h	12h
CON	Vehicle (10% DMSO)	Saline	Sacrifice (𝑛=16)	Sacrifice (𝑛=16)	Sacrifice (𝑛=16)	Sacrifice (𝑛=16)
TC	Vehicle (10% DMSO)	Taurocholate	Sacrifice (𝑛=16)	Sacrifice (𝑛=16)	Sacrifice (𝑛=16)	Sacrifice (𝑛=16)
ZP	Zerumbone 10 mg/kg	Taurocholate				Sacrifice (𝑛=16)
Z	Zerumbone 10 mg/kg	Saline				Sacrifice (𝑛=16)

In the TC group, rats received the vehicle (10% DMSO, 2 mL/kg) administered via femoral vein half an hour prior to retrograde infusion of a freshly prepared 5% sodium taurocholate solution (1 mg/kg). In the CON group, rats received the vehicle administered via femoral vein. After half an hour, the rats were retrograde infused isotonic saline solution (1 mL/kg). In the ZP group, rats received 10 mg/kg zerumbone dissolved in vehicle (10% DMSO v/v) administered via femoral vein half an hour prior to infusion 5% sodium taurocholate solution (1 mg/kg). In the Z group, rats received the same as ZP group except infusion isotonic saline solution instead of taurocholate.