LPS Counter Regulates RNA Expression of Extracellular Proteases and Their Inhibitors in Murine Macrophages

Figure 1

LPS induced RNA expression changes can be determined by StellARray qPCR arrays. RAW 264.7 cell cultures were stimulated with 100 ng/mL LPS or vehicle for 0, 2, 6, and 18 hours, at which time point RNA was harvested for sequential gene expression analysis. LPS stimulation led to a change in gene expression levels up to 512 fold (Il-6). This effect was already evident after two hours and was sustained for at least 18 hours. Pronounced differences in the basal level of expression (0 hours) were observed between genes (e.g., TGF𝛽1 and TGF𝛽2). Data were analyzed by a two-tailed Mann-Whitney test. *𝑃<0.05 between LPS and vehicle-stimulated cultures. 𝑛=4 for all groups.