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Mediators of Inflammation
Volume 2012, Article ID 157894, 9 pages
Research Article

LPS Counter Regulates RNA Expression of Extracellular Proteases and Their Inhibitors in Murine Macrophages

1The Finsen Laboratory, Rigshospitalet, Copenhagen Biocenter 2200 Copenhagen, Denmark
2Biotech Research and Innovation Centre (BRIC), University of Copenhagen, 2200 Copenhagen, Denmark
3Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, 2200, Copenhagen, Denmark
4Section for Metabolic Receptology and Enteroendocrinolgy, Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, 2200 Copenhagen, Denmark

Received 26 September 2011; Revised 5 December 2011; Accepted 21 December 2011

Academic Editor: Stefanie B. Flohé

Copyright © 2012 Andreas Hald et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1: To analyze the effect of LPS on gene expression in RAW 264.7 cells we customized a qPCR StellARray plate from Lonza, Switzerland. The primer pairs included on the plate were specific for the listed genes, which include inflammatory mediators, reference genes, and genes involved in the regulation of extracellular proteolysis.

Table S2: The expression of selected genes in RAW 264.7 cells before and after (2-18 hours) stimulation with LPS. The values present the relative expression following normalization to DNA as described in the materials and methods section.

Table S3: Gene expression in LPS and vehicle stimulated RAW 264.7 cultures was analyzed after 2, 6, and 18 hours. The GPR fold change (Fold) is presented and GPR p-values (p-value) are highlighted in red when below 0.05. The GPR fold change values are provided in this table to indicate how the gene is regulated, but it should be stressed that the GPR p-values are not directly linked to the fold change values.

Figure S1: The graph depicts the expressional changes of genes encoding extracellular matrix components. Gene expression levels are normalized to DNA as previously described. Data were analyzed by a two-tailed Mann-Whitney test. *p<0.05 between LPS and vehicle-stimulated cultures. n= 4 for all groups.

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