Effects of Trauma-Hemorrhage and IL-6 Deficiency on Splenic Immune Function in a Murine Trauma Model

<table>TNF-<i>α</i> productive capacity of splenocytes in response to their stimulation with LPS in wild-type (WT) and IL-6 knockout (IL-6<sup>−/−</sup>) mice in sham animals (group S), after an isolated femoral fracture (group Fx) and after the combination of a femoral fracture and hemorrhage (group TH-Fx). *stat. significance (<svg height="11.375" id="M9" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 70.050003 11.375" width="70.050003" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,21.26,11.112)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,41.624,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,49.784,11.112)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,53.66,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,61.819,11.112)"><use xlink:href="#x35"></use></g>
</svg>) S vs. Fx and TH-Fx, <sup>#</sup>stat. significance (<svg height="11.375" id="M10" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><path d="M619 482q0 -69 -40.5 -119t-96 -72.5t-118.5 -26.5h-44l-70 20l-31 -151q-14 -67 0.5 -83t89.5 -22l-5 -28h-287l8 28q65 7 81.5 22t29.5 83l79 398q12 56 0.5 70.5t-78.5 20.5l7 28h255q108 0 164 -43t56 -125zM524 478q0 141 -146 141q-25 0 -47 -8q-16 -6 -20.5 -13.5
t-10.5 -39.5l-43 -241q37 -13 83 -13q67 0 125.5 45t58.5 129z" id="x1D443"></path></g><g transform="matrix(.017,-0,0,-.017,15.582,11.112)"><path d="M512 -3l-437 233v51l437 233v-58l-378 -200v-2l378 -199v-58z" id="x3C"></path></g><g transform="matrix(.017,-0,0,-.017,30.286,11.112)"><path d="M241 635q53 0 94 -28.5t63.5 -76t33.5 -102.5t11 -116q0 -58 -11 -112.5t-34 -103.5t-63.5 -78.5t-94.5 -29.5t-95 28t-64.5 75t-34.5 102.5t-11 118.5q0 58 11.5 112.5t34.5 103t64.5 78t95.5 29.5zM238 602q-32 0 -55.5 -25t-35.5 -68t-17.5 -91t-5.5 -105
q0 -76 10 -138.5t37 -107.5t69 -45q32 0 55.5 25t35.5 68.5t17.5 91.5t5.5 105t-5.5 105.5t-18 92t-36 68t-56.5 24.5z" id="x30"></path></g><g transform="matrix(.017,-0,0,-.017,38.446,11.112)"><path d="M113 -12q-24 0 -39.5 16t-15.5 42q0 24 16 40.5t40 16.5t40 -16.5t16 -40.5q0 -26 -16 -42t-41 -16z" id="x2E"></path></g><g transform="matrix(.017,-0,0,-.017,42.321,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,50.481,11.112)"><path d="M153 550l-26 -186q79 31 111 31q90 0 141.5 -51t51.5 -119q0 -93 -89 -166q-85 -69 -173 -71q-32 0 -61.5 11.5t-41.5 23.5q-18 17 -17 34q2 16 22 33q14 9 26 -1q61 -50 124 -50q60 0 93 43.5t33 104.5q0 69 -41.5 110t-121.5 41q-53 0 -102 -20l38 305h286l6 -8
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</svg>) Fx versus. TH-Fx, <sup>$</sup>stat. significance (<svg height="11.375" id="M11" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 70.050003 11.375" width="70.050003" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,21.26,11.112)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,41.624,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,49.784,11.112)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,53.66,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,61.819,11.112)"><use xlink:href="#x35"></use></g>
</svg>) WT vs. IL-6<sup>−/−</sup>.</table>

Mediators of Inflammation

fig1

Figure 1

Figure 1: Effects of Trauma-Hemorrhage and IL-6 Deficiency on Splenic Immune Function in a Murine Trauma Model 