Activation of Human Neutrophils by the Anti-Inflammatory Mediator Esenbeckia leiocarpa Leads to Atypical Apoptosis
Figure 3
CHE induces apoptosis in human neutrophils. Freshly isolated human neutrophils were isolated and incubated (10 × 106 cells/mL) with the diluent (SA) or with the indicated concentrations of CHE for 22 h. Apoptosis was evaluated by cytology (a) or by flow cytometry (b) using FITC-annexin-V as described Section 2. Inset in (a): picture of apoptotic (asterisks) or nonapoptotic (arrows) PMNs. Inset in (b): typical results obtained by FACS analysis from one experiments plotted in the corresponding bar graph. Results are means ± SEM (). SA: spontaneous apoptosis (cells incubated with the diluent). * versus SA by Student’s t-test.
