Activation of Human Neutrophils by the Anti-Inflammatory Mediator Esenbeckia leiocarpa Leads to Atypical Apoptosis

Figure 6

CHE induces apoptosis by a caspase-dependent mechanism. Cells (10 × 10⁶ cells/mL) were incubated with (+) or without (−) CHE in the presence (+) or absence (−) of a given caspase inhibitor: z-VAD-FMK (pan-caspase), z-DQMD-FMK (caspase-3), z-VEID-FMK (caspase-6), or z-LEHD-FMK (caspase-9) for 22 h. Apoptosis was evaluated by cytology. Results are means ± SEM (𝑛=4). *𝑃<0.05 versus Ctrl (left column, absence of CHE and inhibitors) and ^#𝑃<0.05 versus CHE by Student’s t-test.