Mediators of Inflammation / 2012 / Article / Fig 1

Research Article

Distinct Proteasome Subpopulations in the Alveolar Space of Patients with the Acute Respiratory Distress Syndrome

Figure 1

Western blots with a polyclonal antibody directed against LMP2 or LMP7 subunits of the immunoproteasome of samples of BAL supernatant and BAL cell pellet lysate obtained from ARDS patients. (a) LMP2 immunoproteasome protein was detected in both BAL supernatant and cell pellet in all ARDS patients. Lanes are identified as follows: Lane 1: 1 μg immunoproteasome (human spleen); Lane 2: cell pellet ARDS patients 1; Lane 3: BAL supernatant ARDS patients 1; Lane 4: cell pellet ARDS patients 2; Lane 5: BAL supernatant ARDS patients 2; Lane 6: cell pellet ARDS patients 3; Lane 7: BAL supernatant ARDS patients 3; Lane 8: cell pellet ARDS patients 4; Lane 9: BAL supernatant ARDS patients 4. (b) LMP7 immunoproteasome protein was detected in both BAL supernatant and cell pellet in all ARDS patients. Lanes are identified as follows: Lane 1: 1 μg immunoproteasome (human spleen); Lane 2: 1 μg 20S standard proteasome (human erythrocyte); Lane 3: cell pellet ARDS patients 1; Lane 4: BAL supernatant ARDS patients 1; Lane 5: cell pellet ARDS patients 2; Lane 6: BAL supernatant ARDS patients 2; Lane 7: cell pellet ARDS patients 3; Lane 8: BAL supernatant ARDS patients 3.
204250.fig.001a
(a)
204250.fig.001b
(b)

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