The Absence of Nrf2 Enhances NF-𝜿B-Dependent Inflammation following Scratch Injury in Mouse Primary Cultured Astrocytes

Figure 3

RT-PCR analysis of mRNA level of proinflammatory cytokines and MMP9. (a) RT-PCR showed that mRNA level of TNF-α, IL-1β, IL-6, and MMP9 was elevated in astrocytes after scratch and was significantly higher in group KO TBI than in group WT TBI. (b–e) Quantitative analysis of RT-PCR results showed that relative mRNA level of proinflammatory cytokines mentioned above and MMP9 was higher in astrocytes after scratch (𝑃<0.01). Such elevation was more severe in group KO TBI than in group WT TBI (𝑃<0.01).(*𝑃<0.01, compared with sham counterpart; ^#𝑃<0.01, compared with WT TBI).

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