Mast Cell-Mediated Inhibition of Abdominal Neutrophil Inflammation by a PEGylated TLR7 Ligand

Figure 1

TLR7 ligand hyposensitization reduces the inflammation of DSS-induced experimental colitis. (a) Experimental protocol of induction of DSS-induced colitis and treatment regimen. WT mice (𝑛=5) received 2% DSS for 7 days. Mice were s.c. treated with 150 nmol 1V136 or vehicle. (b) Disease activity index, (c) percent body weight changes, and (d) MPO activity of colonic tissue was determined as described in Section 2. Data are expressed as means ± SEM and are representative of 2 independent experiments. *Denotes 𝑃<0.05 by Mann-Whitney test. (d) Examples of colons of vehicle- or 1V136-treated colitis mice that were removed and prepared for histological examination. Original magnification is ×100. Calibration bar: 100 μm.

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