Mast Cell-Mediated Inhibition of Abdominal Neutrophil Inflammation by a PEGylated TLR7 Ligand
Figure 2
PEGylated TLR7 ligand reduced neutrophil recruitment in TG-elicited peritonitis. RAW264.7 (a) and primary BMDC (b) were treated 18 h with Tolerimod1 (TLM1), and TNFα and IL-6 in the supernatants were measured by ELISA. (c and d) WT mice ( to 10 per group) were treated daily for three days (-3, -2, and -1) or one day (-3 or -1 day) of s.c. 200 nmol Tolerimod1 (TLM1). Peritonitis was induced on day 0. Total cell number (c) and total neutrophil number (d) recovered in the peritoneal lavage were measured. Data shown are mean ± SEM and are pooled 2 independent experiments that showed similar trends. *Denotes by one way ANOVA with Dunnett’s post hoc testing compared to the vehicle-treated group.
