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Mediators of Inflammation
Volume 2012, Article ID 318621, 9 pages
http://dx.doi.org/10.1155/2012/318621
Research Article

Resolvin D1 Reduces the Immunoinflammatory Response of the Rat Eye following Uveitis

1Department of Ophthalmology, Second University of Naples, 80100 Naples, Italy
2Department of Experimental Medicine, Section of Pharmacology “L. Donatelli”, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy
3Department of Clinical, Public and Preventive Medicine, Second University of Naples, 80138 Naples, Italy

Received 28 August 2012; Revised 14 November 2012; Accepted 14 November 2012

Academic Editor: Fulvio D'Acquisto

Copyright © 2012 Rossi Settimio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated whether the administration of resolvin D1 to rats with endotoxininduced uveitis (EIU) ameliorates the immuno-inflammatory profile of the eye. 24 h after the administration of 200 μg LPS into the footpad of Sprague-Dawley rats, severe changes of the structure of the eye occurred concomitantly with a severe inflammatory and immune response. These latter included strong infiltration of PMN leukocytes CD11b+ T-lymphocytes CD4+ and CD8+ within the eye and a significant release of the cytokines/chemokines TNF-alpha, CXCL8, and RANTES too. Bolus of resolvin D1 (RvD1; 10–100–1000 ng/kg in 200 μL of sterile saline via the tail vein) significantly and dose-dependently (i) reduced the development of the ocular derangement caused by LPS; (ii) reduced the clinical score attributed to EIU; (iii) reduced the protein concentration and myeloperoxidase activity (MPO) in aqueous humor (AqH); and (iv) reduced neutrophils, T-lymphocytes, and cytokines within the eye.