oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21
Figure 2
Protein expression of CCL21 and CCR7 on carotid plaque and aortic tissue. (a) Analysis of the CCL21 expressing structures in human aortic tissue. In the upper part the blue staining represents the cell nuclei (DAPI) and the red staining represents CCL21, positive structures (α-CCL21/6Ckine-Alexa488, pseudocolored). Scale bar 10 μm. To examine the vessel wall as CCL21 expressing structures (lower part) in human aorta tissue endothelial cells were stained with a PECAM-1 antibody (green, α-PECAM-1-Cy3, pseudocolored). The red staining represents CCL21 (α-CCL21/6Ckine-Atto594). The merged picture shows a co-localization of CCL21 and PECAM-1 in the vessel wall of the human aorta. Scale bar 50 μm. (b) The representative pictures show differences in the expression level of CCL21 (red, α-CCL21/6Ckine-Atto594) in aorta and carotid plaque tissue. A decreased CCL21-protein expression in carotid plaque compared to aorta tissue was shown by immunofluorescence microscopy. CCL21 is primarily expressed by the vasa vasorum of the vessel. Scale bar 50 μm. (c) A decreased CCR7 expression (green, α-CCR7-FITC) on CD4 positive cells (red, α-CD4-Atto594) was detectable in plaque tissue compared to aorta tissue. Arrows indicate the cells. Scale bar 20 μm. (d) Also a decreased CCR7 expression (green, α-CCR7-FITC) on CD83, positive cells (red, α-CD83-Atto5994) in aorta and plaque tissue is shown in the representative pictures. Arrows indicate the cells. Scale bar 20 μm.
(a) Analysis of the CCL21, expressing structures in human aortic tissue
(b) Localisation of CCL21, positive cells in aortic tissue and carotis plaque
