oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21

<table class="table-group" width="800px"><tr class="fig-image" id="a"><td><img alt="320953.fig.004a" src="https://static.hindawi.com/articles/mi/volume-2012/320953/figures/320953.fig.004a.jpg"/></td></tr><tr class="fig-caption"><td align="center"><table><tr><td><b>(a) </b>mRNA expression of CCL21</td></tr></table></td></tr><tr class="fig-image" id="b"><td><img alt="320953.fig.004b" src="https://static.hindawi.com/articles/mi/volume-2012/320953/figures/320953.fig.004b.jpg"/></td></tr><tr class="fig-caption"><td align="center"><table><tr><td><b>(b) </b>Protein expression of CCL21</td></tr></table></td></tr><tr class="fig-image" id="c"><td><img alt="320953.fig.004c" src="https://static.hindawi.com/articles/mi/volume-2012/320953/figures/320953.fig.004c.jpg"/></td></tr><tr class="fig-caption"><td align="center"><table><tr><td><b>(c) </b>Quantification of the fluorescence intensity</td></tr></table></td></tr></table>

<table>mRNA expression of CCL21/19 and protein expression of CCL21 on HMECs-1 after stimulation with oxLDL. (a) Fold increase in mRNA expression levels of CCL21/19 in human microvascular endothelial cells after 48 h of stimulation with oxLDL (10 <i>μ</i>g/mL) analyzed by real-time PCR. *<svg height="11.375" id="M57" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,15.582,11.112)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,30.286,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,38.446,11.112)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,42.321,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,50.481,11.112)"><use xlink:href="#x35"></use></g>
</svg>, (<svg height="11.175" id="M58" style="vertical-align:-0.20474pt" version="1.1" viewbox="0 0 36.237499 11.175" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>) versus unstimulated controls. (b) Corresponding protein expression of CCL21 measured by immunofluorescence analysis. The green staining represents CCL21 positive structures (<i>α</i>-CCL21/6Ckine-Alexa488), red represents the plasma membrane (Vybrand DiD). Scale bar 10 <i>μ</i>m. (c) The fluorescence intensity was quantified showing a significant change for oxLDL. *<svg height="11.375" id="M59" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, (<svg height="11.125" id="M60" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 36.237499 11.125" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,10.862)"><use xlink:href="#x1D45B"></use></g><g transform="matrix(.017,-0,0,-.017,13.305,10.862)"><use xlink:href="#x3D"></use></g><g transform="matrix(.017,-0,0,-.017,28.008,10.862)"><use xlink:href="#x38"></use></g>
</svg>) versus unstimulated control.</table>

Mediators of Inflammation

oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21

Figure 4