BAY 11-7082 Is a Broad-Spectrum Inhibitor with Anti-Inflammatory Activity against Multiple Targets
Figure 5
Effect of BAY on the activation of the IRF-3 pathway. (a left panel and b) Levels of NO by the Griess assay and PGE2 by EIA from culture supernatants of RAW264.7 cell treated with BX795 (5 μM) or AG490 (20 μM) in the presence or absence of LPS (1 μg/mL) for 24 h. (a right panel) Levels of COX-2 and iNOS mRNA by real-time PCR using LPS-treated RAW274.7 cells pretreated with BX795 (5 μM) for 6 h. (c, d, f, and g) Translocated or phosphorylated levels of IRF-3, STAT-1 or JAK2 from nucleus (c and f) or total lysates (d and g) from RAW264.7 cells treated with BAY (15 μM) in the presence or absence of LPS (1 μg/mL) for indicated times, evaluated by immunoblotting. (e) HEK293 cells cotransfected with IFN-β-promoter-1-Luc construct (1 μg/mL) and β-gal (as a transfection control) were treated with BAY in the presence or absence of TBK1 (1 μg/mL). Luciferase activity was measured using a luminometer. Relative intensity (c) was calculated by densitometric scanning. * and ** compared to the control.
