Activated Protein C Induces Endoplasmic Reticulum Stress and Attenuates Lipopolysaccharide-Induced Apoptosis Mediated by Glycogen Synthase Kinase-3β
Figure 1
APC attenuated LPS-induced apoptosis in HUVECs. Flow cytometric analysis of cultured HUVECs continuously exposed to 10 ng/mL LPS for 24 h revealed a high percentage of apoptotic cells (a). Subsequently, LPS-stimulated HUVECs were treated with 150 nM APC for 0, 6, 12 and 24 h ((a), (b), (c), and (d)). APC inhibition of cell apoptosis was detected following 6 h exposure ((b), (e); ) and then generally decreased. Flow cytometric analysis of PI-Annexin V-FITC staining revealed that 150 nM APC treatment markedly attenuated LPS-induced apoptosis in HUVECs.
