Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE2 Production by Suppression of the AP-1/p38 Pathway
Figure 3
Effect of peptide K5 on the activation of signaling events upstream of AP-1 activation. (a) and (b) Phosphoprotein or total protein levels of IκBα, ERK, p38, JNK, MKK3/6, TAK1, IRAK1, and β-actin from cell lysates were determined by Western blot analysis using phosphospecific or total protein antibodies. (c) Levels of members of the AP-1 family (p-Fra-1, c-Jun, and c-Fos) in the nuclear fraction were determined by Western blot analysis using antibodies against total protein. (d) HEK293 cells cotransfected with plasmid constructs NF-κB-Luc, or AP-1-Luc (1 μg/mL each) and β-gal (as a transfection control) were treated with SB203580 (25 μM) in the presence or absence of TNF-α (20 ng/mL) for NF-κB activation or PMA (10 ng/mL) for AP-1 activation. Luciferase activity was measured using a luminometer. * compared with controls.
