Mediators of Inflammation / 2012 / Article / Fig 1

Research Article

Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells

Figure 1

Effects of concentrated ethanol extract of Edelweiss callus cultures (ECC55) on UV-induced keratinocyte damage and intracellular nitric oxide (NO) production. Fluorescent microphotographs of the control untreated keratinocytes (a), keratinocytes 1 h after irradiation by solar simulating UVA+UVB (b), and keratinocytes pretreated with 25 μg/mL of ECC55 for 30 min and then subjected to UVA+UVB exposure (c). Quantification of intracellular fluorescence of NO-specific fluorescent probe DFA-DA (d). Keratinocyte vitality assessed by MTT test. Error bars in all figures correspond to S.D. *P < 0.05 versus sham-irradiated control; §P < 0.05 versus UV irradiation without ECC55; P < 0.01 versus sham-irradiated control; ¥P < 0.01 versus UV irradiation without ECC55.

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