Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells
Figure 4
Effects of concentrated ethanol extract of Edelweiss callus cultures (ECC55) on Trichostatin A-induced cellular senescence and molecular markers of apoptosis, cell cycle, and proliferation. (a)–(d). Phase contrast microphotographs of cultivated human keratinocytes: untreated control (a), treated with Trichostatin A alone (b), treated with ECC55 alone (c), and treated with a combination of Trichostatin A and ECC55 (d). (e) Western blots and their densitometry showing p21 expression upon treatment with 100 μg/mL ECC55 alone, Trichostatin A (TSA) alone, or TSA combined with increasing concentrations of ECC55 (5–100 μg/mL). (f) The same for proliferation-connected nuclear antigen (PCNA). (g) The same for p53 and (h) for caspase 3. Error bars in all figures correspond to S.D. *P < 0.05 and **P < 0.01 versus untreated control; †P < 0.01 and ‡P < 0.001 versus TSA-treated keratinocytes.
