Prevention of LPS-Induced Acute Lung Injury in Mice by Progranulin
Figure 6
PGRN/TNFR2 interaction was crucial for the protective effect of PGRN on LPS-induced ALI. Groups of mice were pretreated with neutralizing antibodies to TNFR1 or TNFR2, respectively. Twenty-four hours later, the mice were challenged with LPS, followed by administration of PGRN. The total cells (a), proinflammatory cytokines (b), albumin (c), and IgM (d) in BAL fluid was detected at day 3 after LPS challenge. The histological evaluation of lung sections (e) and lung injury score (f) were also analyzed at day 3 after LPS challenge. Data are represented as mean ± standard deviation of one experiment consisting of three replicates. *.
