Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor

Figure 1

PAFR antagonists reduce melanoma growth associated with a massive number of apoptotic cells. The treatment of C57Bl/6 mice with PAFR antagonists showed a significant reduction in the melanoma growth (10³ Tm1 viable cells) induced by apoptotic cells (10⁶  γ-irradiated Tm1 cells, (a) and (b)) but not by carrageenan (0.1% (c)). The animals were treated with PBS or PAFR antagonists (WEB2170 (WEB, (a)) or PCA4248 (PCA, (b) and (c)) 5 mg/kg body weight)) by peritumor daily injections during 5 days. All experiments were performed using groups of 5 to 10 mice, which were considered harboring tumors when the subcutaneous mass reached 20 mm³ (palpable tumor). *Denotes statistical significance (𝑃<0.05) using nonpaired nonparametric Student’s t-tests.

(a)

(b)

(c)