Mediators of Inflammation / 2012 / Article / Fig 1

Clinical Study

Alterations in Monocyte CD16 in Association with Diabetes Complications

Figure 1

Flow cytometry gating strategy. Flow cytometry data obtained from a representative sample is shown. (a) Peripheral blood cells were first gated based on forward scatter (FSC-A)/side scatter (SSC-A), with exclusion of dead cells. (b) The events were then visualized using FSC-A/FSC-H dot plot, and the singlets (single cells) were gated. (c) Leukocytes were identified by their positive staining with CD45. (d) Monocytes were then defined as the CD14+ cells within the CD45+ leukocyte population. (e) The final gating was based on the CD marker of interest within the CD45+CD14+ cell population. (f) Necrotic cells in the whole blood sample were detected by Propidium Iodide (PI) staining.