Anti-Inflammatory Effects of Adrenomedullin on Acute Lung Injury Induced by Carrageenan in Mice

Figure 5

Effect of adrenomedullin (AM) on proinflammatory cytokine expression and release. Lung sections taken from CAR-treated mice showed positive staining for TNF-α and IL-1β (b and f, resp.). There was a marked reduction in the immunostaining for TNF-α and IL-1β in the lungs from CAR-treated mice treated with AM (c and g, resp.). No staining for either TNF-α (a) or IL-1β (e) in lung tissues obtained from the sham group was detected. The figure is representative of at least 3 experiments performed on different experimental days. Moreover, pleural injection of CAR caused an increase in exudate levels of TNF-α (d) and IL-1β (h). AM significantly reduced TNF-α and Il-1b levels (d and h, resp.). Finally IL-10 were measured in the lung exudates. In CAR-treated mice, however, significant decreases (𝑃<0.01) in the levels of IL-10, in comparison to values obtained inAM-treated mice, were detected (i). Data are means ± s.e.m. of 10 mice for each group. ^∗𝑃<0.01 versus sham group. ^∘𝑃<0.01 versus CAR.