Anti-Inflammatory Effects of Adrenomedullin on Acute Lung Injury Induced by Carrageenan in Mice

Figure 6

Effect of adrenomedullin (AM) on IκB-α degradation, nuclear NF-κB p65 quantification, IKK-α, PKA, and phospho-NF-κB (ser536). Basal level of IκB-α was detected in lung samples from sham-treated animals, whereas IκB-α levels were substantially reduced in lung tissues obtained from vehicle-treated animals at 4 h after CAR injection (a, a1). AM treatment prevented CAR-induced IκB-α degradation (a, a1). NF-κB p65 levels in the lung nuclear fractions were significantly increased at 4 h after CAR injection compared to the sham-treated mice (b, b1). AM treatment significantly reduced the levels of NF-κB p65 (b, b1). AM significantly reduced the levels of IKK alpha (e, e1) and the phosphorylation of p65 on Ser536 (c, c1). Whereas prevented CAR-induced PKA decreases (d, d1). A representative blot of lysates obtained from 5 animals per group is shown and densitometry analysis of all animals is reported. The results in panel (a1, b1, c1, d1 and e1) are expressed as mean ± s.e.m. from 𝑛=5/6 lung tissues for each group. ^∗𝑃<0.01 versus sham group. ^∘𝑃<0.01 versus CAR.