CXCL1-Triggered Interaction of LFA1 and ICAM1 Control Glucose-Induced Leukocyte Recruitment during Inflammation In Vivo
Figure 2
Leukocyte adhesion (number of adherent cells/mm2) in mouse cremaster muscle venules and leukocyte transmigration (perivascular neutrophils/mm2 surface area) in cremaster muscle whole mounts in response to glucose during TNFα-induced inflammation. (a) Leukocyte adhesion was observed in TNFα-stimulated cremaster muscle venules of wild-type mice (17 venules in 5 mice) before and during 15 minutes after intravenous injection of 0.5 g/kg glucose and compared to injection of normal saline (9 venules in 4 mice). (b) Giemsa-stained whole mounts of TNFα-treated cremaster muscles of WT mice were analyzed for the number of perivascular neutrophils after injection of glucose or normal saline. Representative micrographs are depicted in Figures 2(c) and 2(d). Reference bar is presented in Figure 2(d). Arrows point to extravasated leukocytes. All values are presented as mean ± SEM. Significances are indicated by the asterisks (* versus WT control mice).
