CXCL1-Triggered Interaction of LFA1 and ICAM1 Control Glucose-Induced Leukocyte Recruitment during Inflammation In Vivo

Table 3

Hemodynamic and microvascular parameters of cremaster muscle venules in ICAM1-KO, LFA1-KO, and PTx-pretreated or KC-coinjected WT mice before and after injection 0,5 g/kg glucose in the trauma model. Vessel diameter, centerline velocity, and wall shear rate are displayed before and after the intravenous administration of glucose. Experiments are presented as mean ± SEM; n.s.: not significant stating no differences of hemodynamic parameters among the different groups and during the time course after glucose/saline injection.

	Mice	Venules	Diameter	Centerline velocity	Wall shear rate	Systemic leukocyte counts
	𝑁	𝑛	(𝜇m)	(𝜇m/s)	(s^−1)	(/𝜇L)
WT mice
Pre-glucose	16	64	28 ± 1	2200 ± 100	1900 ± 100	6500 ± 100
5′ after glucose	16	64	28 ± 1	2300 ± 100	2000 ± 200	6200 ± 200
15′ after glucose	16	64	28 ± 1	2400 ± 100	2000 ± 100	6400 ± 100
			n.s.	n.s.	n.s.	n.s.
Coinjection with KC in WT mice
Pre-glucose	4	16	27 ± 1	2200 ± 100	2200 ± 100	5900 ± 300
5′ after glucose	4	12	27 ± 1	2300 ± 100	2200 ± 100	6000 ± 200
15′ after glucose	4	14	27 ± 1	2200 ± 100	2000 ± 100	6000 ± 200
			n.s.	n.s.	n.s.	n.s.
PTx-pretreatment in WT mice
Pre-glucose	4	16	29 ± 1	2200 ± 100	2000 ± 100	5700 ± 300
5′ after glucose	4	15	29 ± 1	2300 ± 200	2000 ± 200	5200 ± 500
15′ after glucose	4	16	29 ± 1	2400 ± 100	2000 ± 100	5300 ± 300
			n.s.	n.s.	n.s.	n.s.
LFA1-KO mice
Pre-glucose	4	25	28 ± 1	2500 ± 100	2200 ± 100	7600 ± 500
5′ after glucose	4	19	28 ± 1	2400 ± 100	2200 ± 100	8000 ± 300
15′ after glucose	4	20	28 ± 1	2500 ± 100	2200 ± 100	8100 ± 300
			n.s.	n.s.	n.s.	n.s.
ICAM1^−/− mice
Pre-glucose	4	22	27 ± 1	2300 ± 100	2300 ± 100	6000 ± 400
5′ after glucose	4	18	27 ± 1	2500 ± 100	1800 ± 100	6200 ± 500
15′ after glucose	4	21	27± 1	2400 ± 100	2300 ± 100	6100 ± 400
			n.s.	n.s.	n.s.	n.s.