The Impact of Various Reactive Oxygen Species on the Formation of Neutrophil Extracellular Traps
Figure 3
Flow cytometry analysis of intracellular ROS production by neutrophils after exposure to various inhibitors. Freshly isolated human neutrophils were incubated with or without inhibitors (10 μM Rotenone, 10 μM Dinitrophenol, 500 nM FCCP, 20 μM DPI, 38 μM Aroclor, 10 μM DETC, 200 μM Dipyrone, and 200 μM Aminopyrine) for 30 min at 37°C. Cells were loaded with DHR 123, and the samples were stimulated with 4 μM PMA. The intracellular production of ROS was assessed by using flow cytometry. (a) Geometrical mean (GeoMean) values of the fluorescence intensities normalized to the PMA-induced ROS production in the absence of inhibitors (Medium). Data show mean ± SD from four independent experiments, *: as compared to the PMA-stimulated sample without inhibitor (Medium). (b) Representative histogram showing the fluorescent intensities of unstimulated neutrophils (without PMA), PMA-stimulated neutrophils without inhibitor (Medium), and, as an example for an inhibitory effect, PMA-stimulated neutrophils after exposure to DPI. Data are from one experiment representative for three independent experiments.
