Adipose Tissue-Specific Deletion of 12/15-Lipoxygenase Protects Mice from the Consequences of a High-Fat Diet
Fat-specific 12/15-LO-deficient mice exhibit improved glucose metabolism when fed a high-fat diet. (a) Body weight, nonfasted serum insulin, and nonfasted serum insulin/nonfasted blood glucose ratio were measured at the end of the 16-week feeding regimen. Fasted blood glucose was measured in overnight fasted mice at 15 weeks of the feeding regimen. Serum insulin levels were measured by ELISA and tail vein blood glucose by glucometers. (b)-(c) Intraperitoneal glucose tolerance test (GTT) and insulin tolerance test (ITT) were performed in mice after 15 weeks of feeding, and blood glucose measurements were measured at the indicated time points. Areas under the curves (AUC) are presented for GTT and ITT. All data represent the means ± SEM; = 3–6. , , versus control, unless otherwise indicated. Chow: chow diet; HFD: high-fat diet; wt: wild type 12/15-.