Adipose Tissue-Specific Deletion of 12/15-Lipoxygenase Protects Mice from the Consequences of a High-Fat Diet
Adipose tissue from fat-specific 12/15-LO-deficient mice exhibits decreased macrophage infiltration and may be more adipogenic and lipogenic. (a) mRNA expression of key macrophage markers was measured by RT-PCR in stromal vascular cells (SVCs) isolated from epididymal adipose tissue. (b) Immunohistochemistry for Mac2 expression was performed in epididymal adipose tissue, and Mac2 staining was quantified. Scale bar = 50 μM. (c) Protein measurements of cleaved caspase-3 were performed from isolated epididymal adipocytes and quantified. Representative western blots are shown, and separate panels for each antibody represent the same exposure from the same gel. (d) mRNA measurements of key adipogenic and lipogenic genes were examined by RT-PCR in epididymal adipose tissue from mice. All mRNA and protein data were normalized to total actin, and the fold changes in expression were calculated relative to control. All data represent the means ± SEM; = 3–6. , , versus control, unless otherwise indicated. Chow: chow diet; HFD: high-fat diet; wt: wild type 12/15-.