Adipose Tissue-Specific Deletion of 12/15-Lipoxygenase Protects Mice from the Consequences of a High-Fat Diet
Fat-specific 12/15-LO-deficient mice exhibit improvements in pancreatic islet health. (a) Immunohistochemistry for insulin expression on pancreatic sections from mice after the 16-week feeding regimen was analyzed for average islet area, average islet area per pancreas area, and average insulin staining per islet; = 3–5; Chow: chow diet; HFD: high-fat diet; wt: wild type 12/15-. (b) mRNA measurements of key proinflammatory genes were examined by RT-PCR in isolated pancreatic islets from mice after the 16-week feeding regimen; = 6–8; HFD: high-fat diet; wt: wild type C57BL/6J. All mRNA data were normalized to total actin, and the fold changes in expression were calculated relative to control. All data represent the means ± SEM. and versus control, unless otherwise indicated.