Research Article

Flavonoid Naringenin: A Potential Immunomodulator for Chlamydia trachomatis Inflammation

Figure 1

Naringenin downregulates inflammatory mediators in C. trachomatis infected mouse J774 macrophages. Macrophages (106 cells/mL) were seeded in 24-well plates and were either infected with live C. trachomatis (105 IFU/well) or LPS at 1 μg/mL. After 2-day infection, naringenin at 0.01 to 10 μg/mL was added to cell cultures and the production levels of cytokines (a) and chemokines (b) were quantified in supernatants collected 2 days later employing multiplex ELISA. ***indicates significant difference ( ) between C. trachomatis treated cells and those treated with various concentrations of naringenin using the two-tailed unpaired Student’s -test. Each bar represents the average of samples run in duplicates and the data are representative of three separate experiments.
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(a)
102457.fig.001b
(b)