Flavonoid Naringenin: A Potential Immunomodulator for Chlamydia trachomatis Inflammation
Figure 3
Naringenin downregulates the expression levels of CD80, TLR2, and TLR4 in C. trachomatis infected mouse J774 macrophages. Macrophages (106 cells/mL) were seeded in 24-well plates and infected with C. trachomatis (105 IFU/well) or left uninfected. After 2-day infection, 1 μg/mL naringenin was added to cell cultures and 2 days later samples were analyzed by flow cytometry as described in Section 2. Shown are the expression shifts of TLR2 (a), CD80 (b), TLR4 (c), and CD86 (d) and their mean fluorescence intensity (MFI) (e) before and after infection of macrophages with C. trachomatis (CT) in the presence and absence of naringenin. is considered significant as compared to untreated cells (J774) and to cells treated with CT or CT + naringenin. The data are representative of two separate experiments.
