Flavonoid Naringenin: A Potential Immunomodulator for Chlamydia trachomatis Inflammation
Figure 5
C. trachomatis employs the p38 MAPK pathway for production of inflammatory mediators in mouse J774 macrophages. Macrophages (106 cells/mL) were seeded in 24-well plates in the presence and absence of the SB-203358 p38 inhibitor for 24 h, after which they were left uninfected or infected with live C. trachomatis (105 IFU/mL). After 3 days following infection with C. trachomatis (CT), supernatants were collected and the levels of inflammatory mediators were determined by single ELISAs. is considered significant when compared to untreated cells (J774) and to cells treated with CT or CT + SB. Data shown is an average of duplicate run representative of two separate experiments.
