Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
Figure 6
Treatment with 1.4% ISO inhibits pulmonary cell apoptosis in ZY-challenged mice. Western blot analysis for expression of procaspase-8 (a), cleaved caspase-8 (c), procaspase-3 (e), cleaved caspase-3 (g), Bax (i), and Bcl-2 (k); protein bands were densitometrically quantified and relative amounts of procaspase-8 (b), cleaved caspase-8 (d), procaspase-3 (f), cleaved caspase-3 (h), Bax (j), and Bcl-2 (l) are shown; histologic sections were stained with anti-Bax antibody ((m); 40x or 400x magnification) and anti-Bcl-2 antibody ((n); 40x or 400x magnification). The respective densitometric analysis of protein bands is from three separate experiments. Immunohistochemistry figures are representative of at least three experiments performed on different experimental days. The animals were treated as described in Figure 1. Data are mean ± SD ( mice per group). * versus sham; # versus ZY + vehicle.
