127017.fig.001a
(a)
127017.fig.001b
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127017.fig.001c
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127017.fig.001d
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127017.fig.001e
(e)
Figure 1: Gonococcus uptake by murine macrophage cell line: fluorescence micrograph of murine macrophage cell line RAW 264.7 incubated with GFP-expressing Neisseria gonorrhoeae (green) variant P9-17 for (a) 1 h, (b) 2 h, (c) 3 h, and (d) orthogonal view of the intracellular gonococci (green spots); a midplane -section of height 1.25 μm is shown. Phase contrast denotes cell boundaries. Nuclei are in red. (e) Gentamicin protection assay for infection of RAW cells with N. gonorrhoeae variant P9-17 ( ).