Paraoxonase-1 Inhibits Oxidized Low-Density Lipoprotein-Induced Metabolic Alterations and Apoptosis in Endothelial Cells: A Nondirected Metabolomic Study

Figure 5

(a) Western blot analyses for caspase 9; (b) percentage of apoptotic cells; (c) relationship between total peroxide concentrations and the percentage of apoptotic cells in HUVEC cell homogenates (𝑛=3, for each experiment). Endothelial cells were incubated over 24 h with 50 mg/L isolated human LDL (L); 50 mg/L oxidized LDL (O); 50 mg/L oxidized LDL + 40 mg/L HDL from wild type mice (OH); 50 mg/L oxidized LDL + 40 mg/L HDL from PON1^(−/−) mice (KO); or with serum-free media as controls (C). MW: molecular weight marker. ^∗𝑃<0.01 with respect to C; ^†𝑃<0.05 with respect to OH; ^‡𝑃<0.01 with respect to O.

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