Induction of Tumor Necrosis Factor (TNF) Release from Subtypes of T Cells by Agonists of Proteinase Activated Receptors
Induction of upregulated expression of TNF mRNA in purified T cells by thrombin and trypsin. (a) Cells were incubated with various concentrations of thrombin (Th, μg/mL), trypsin (Ts, μg/mL), SFLLR-NH2 (SF, μM), and TFLLRN-NH2 (TF, μM) for 30 min, 2 h or 6 h at 37°C. (b) Cells were incubated with Th (μg/mL) and Ts (μg/mL) in the presence or absence of their inhibitors, respectively for 6 h at 37°C. Values shown are mean ± SEM for four to six independent experiments from different donors. * compared with the response to medium alone control. † compared with the response to the corresponding proteinase alone. Hir = hirudin (U/mL), TI = SBTI (μg/mL), and SCH = SCH 79797 (μM).