p38/AP-1 Pathway in Lipopolysaccharide-Induced Inflammatory Responses Is Negatively Modulated by Electrical Stimulation

Figure 5

Effect of SB203580 on the expression of COX-2 and TNF-𝛼 mRNA and the production of TNF-𝛼 and PGE₂. ((a) and (b)) The levels of COX-2 and TNF-𝛼 mRNA were determined using semiquantitative RT-PCR or real-time PCR. (a) The levels of TNF-𝛼 and PGE₂ were determined using ELISA and EIA on culture supernatants from RAW264.7 cells that had been treated with SB203580 (20 𝜇M) and LPS (1 𝜇g/mL) for 6 h (TNF-𝛼) or 24 h (PGE₂) h. *𝑃<0.05 and **𝑃<0.01 compared to control.

(a)

(b)