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Mediators of Inflammation
Volume 2013, Article ID 193484, 9 pages
http://dx.doi.org/10.1155/2013/193484
Research Article

Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model

1Postgraduate Program in Pulmonology, Federal University of Rio Grande do Sul (UFRGS), 90040-060 Porto Alegre, RS, Brazil
2Methodist University (IPA), 906301-170 Porto Alegre, RS, Brazil
3Thoracic Surgery Department, Laboratory of Airways and Lung, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2.350, 90035-903 Porto Alegre, RS, Brazil
4Lutheran University of Brazil (ULBRA), 92425-900 Canoas, RS, Brazil
5Department of Cardiopneumology, Division of Thoracic Surgery, Laboratory of Medical Investigation (LIM 61), Heart Institute (InCor), Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, 05403-000 São Paulo, SP, Brazil
6Santo Antônio Children’s Hospital, 90020-090 Porto Alegre, RS, Brazil

Received 10 June 2013; Revised 21 September 2013; Accepted 7 November 2013

Academic Editor: Tânia Fröde

Copyright © 2013 Luiz Alberto Forgiarini Junior et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. The aim of this study was to evaluate the use of liquid perfluorocarbon (PFC) as an adjuvant substance for lung preservation and assess its role in pulmonary protection after transplantation. Methods. Seventy-two rat lungs were flushed with low-potassium dextran (LPD) solution and randomized into three main groups: control with LPD alone and experimental with 3 (PFC3) and 7 mL/kg (PFC7) of endobronchial PFC instilled just after harvest. Each group was divided into four subgroups according to preservation time (3, 6, 12, and 24 hours). Afterwards, we performed lung transplantation using rat lungs preserved for 12 hours with LPD alone or with 7 mL/kg of endobronchial PFC. Results. There was a significant increase in oxidative stress in the control group at 6 h of cold ischemic time compared with the PFC3 and PFC7 groups. The apoptotic activity and NF- B expression were significantly higher in the control group compared with the PFC groups at 3, 12, and 24 h of cold preservation. After transplantation, the NF- B, iNOS, and nitrotyrosine expression as well as caspase 3 activity were significantly lower in the PFC groups. Conclusion. The use of endobronchial PFC as an adjuvant to the current preservation strategy improved graft viability.