Oxidized LDL Induces Alternative Macrophage Phenotype through Activation of CD36 and PAFR
Figure 3
oxLDL induces macrophages differentiation towards M2 phenotype. Murine bone marrow-derived cells were differentiated to macrophages (BMDM) with L929 supernatant for six days in the presence of oxLDL. Cells were treated with oxLDL (20 μg/mL) on the first day of culture and supplemented every two days. The mRNA expression of IL-10, arginase-1 (Arg-1), mannose receptor (MR), PPARγ, IL-12p40, and iNOS was assessed by real-time PCR. Data are presented as mean ± SEM of independent experiments and expressed in fold change. * comparing oxLDL-treated with nontreated cells.
