The Serum IL-6 Profile and Treg/Th17 Peripheral Cell Populations in Patients with Type 1 Diabetes
Figure 1
Comparison between percentage (a) and absolute number (b) of CD4+Foxp3+ T cells in peripheral blood of diabetic type 1 patients and control group. Fresh, resting PBMCs from diabetic type 1 patients (DM1) and healthy individuals (C) were stained with antibodies against CD4 and Foxp3 molecules and analyzed using flow cytometry. The gate was set on CD4+ lymphocytes according to forward scatter and staining with CD4-Pe/Cy5 and then the percentage of CD4+Foxp3+ T cells in CD4+ gate was determined. Data were calculated with Mann Whitney test and presented as medians and minimum/maximum. The median percentage of CD4+Foxp3+ T regulatory cells in DM1 and control group was 1.15 (0.6/3.0) and 4.8 (3.8/8.8), respectively. The median absolute number of CD4+Foxp3+ T regulatory cells in DM1 and control group was 0.39 (0.21/1.2) and 1.96 (1.3/3.43), respectively. *The percentage of cells among peripheral blood lymphocytes.
