IL-22 is downregulated in lungs from bleomycin-treated mice. (a) Total protein level of IL-22 in lung homogenates was determined by western blotting and analyzed by densitometry compared to GADPH expression. The same amounts of total protein are loaded in each lane. Experiments were performed twice with similar results, with the same amounts of total protein loaded in each lane. (b) Representative photomicrographs of lung sections were obtained from mice treated with BLM or saline (week 3). IL-22-positive cells were examined by immunohistochemistry. μm. ; . Data presented in (a) are representative of results from two independent experiments with five mice in each group. Values are shown as .