ATP Is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro

Figure 3

IL-1β mediates gap junctional communication induced by pro-inflammatory molecules. (a) Effect of 20 ng/mL IL-1 receptor agonist (IL-1ra) over LY transfer in EOC20 cells treated with TNF-α plus ATP for 3.5 h or with TNF-α/IFN-γ for 9 h. ^#𝑃<0.05 between indicated treatments. Each bar represents the mean ± SEM, 𝑛=4. (b) The effect of 50 μM 18-β-glycyrrhetinic acid (β-GA) acutely applied or treatment with 10 ng/mL interleukin-6 (IL-6) or 300 μM oxidized ATP (oATP) over LY transfer in EOC20 cells treated with TNF-α/IL-1β for 9 h is also shown. Each bar represents the mean ± SEM, 𝑛=4, and corresponds to the percentage of incidence of dye coupling under control conditions (dashed line). ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus control condition.

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