IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo
Figure 2
Surface receptor expression of polarised macrophages and intracellular IRF5 staining. Macrophages were in vitro differentiated with GM-CSF or M-CSF for eight days and then stimulated with LPS for 24 h. FACS samples were collected before and after stimulation. (a) and (b) Samples were stained for the expression of F4/80, CD206, MHC II, and IRF5. (c) Macrophages were stained for intracellular IRF5 and staining in unstimulated cells was quantified by mean fluorescence intensity (MFI). Error bars represent the standard error for . Statistical analysis was performed by one-tailed Mann-Whitney test. **.
