IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo
Figure 3
Macrophage populations and IRF5 expression at the site of inflammation in a mouse model of arthritis. Mice were immunised with mBSA in complete Freud’s adjuvant prior to intra-articular injection of mBSA or PBS. Knees were collected at day two of disease. (a) Schematic of the experimental set-up for antigen-induced arthritis. (b) Samples from three independent experiments were stained for flow cytometry with antibodies against CD45, CD11b, F4/80, CD206, and MHC II. (c) IRF5 FACS staining was quantified calculating the mean fluorescence intensity in knees of three wild type mice. (d) Total RNA was isolated from knees of three independent experiments and analysed by real-time PCR for expression of Irf5, iNOS, and Fizz1. Statistical analysis was performed throughout by one-tailed Mann-Whitney test. *; **; ***; ****.
