Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4
Figure 5
The effects of berberine on protein expression of eNOS, p-eNOS, Akt, p-Akt, AMPK, and p-AMPK in HUVECs exposed to palmitate. HUVECs were cultured in RPMI-1640 containing 0.5 mmol/L palmitate and treated with 1.25, 2.5, and 5 μmol/L berberine for 24 h. Total protein was prepared and separated by SDS-PAGE. Expression and relative quantification of eNOS, Akt, and AMPK and phosphorylation of eNOS, Akt, and AMPK protein levels were expressed relative to the amount of actin. Control group was not stimulated with 0.5 mmol/L palmitate. Data are mean ± S.E.M. All presented results are representative of at least 3 independent experiments. * versus control group, # versus palmitate treated group.
