Pharmacological Inhibition of p38 Mitogen-Activated Protein Kinases Affects KC/CXCL1-Induced Intraluminal Crawling, Transendothelial Migration, and Chemotaxis of Neutrophils In Vivo
Figure 2
Effect of SB203580 on KC-induced neutrophil adhesion and emigration. (a) Time course of the number of adherent neutrophils (cells/100 μm venule) triggered by KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). ** indicates significant difference () from the control without SB203580 (t-test). (b) Time course of the number of emigrated neutrophils (cells/235 × 208 μm2 field) triggered by KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). ** indicates significant difference () from the control without SB203580 (t-test). (c) Representative images from intravital video microscopy showing a postcapillary venule and the surrounding cremasteric tissue with adherent and emigrated neutrophils triggered by KC for 60 min and in the absence (left panel, control) and in the presence of SB203580 (right panel, SB203580).
