Plasmacytoid Dendritic Cell Response to CpG ODN Correlates with CXCL16 Expression and Is Inhibited by ox-LDL
Figure 2
Digestion of CXCL16 on the surface of pDCs reduces cytokine production induced by D-ODN. (a) PBMC (4 × 106/mL) were preincubated in the absence or presence of O-sialoglycoprotein endopeptidase (25 μg/mL) for 30 min, fixed, and stained for CXCL16 expression. (b) Cells treated as in (a) were washed and then stimulated with 3 μM D-ODN for 24 h. Cytokine production was assessed from culture supernatants using ELISA. Response of 6 individual donors is shown.
