Research Article

Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum

Figure 2

GLP-1 restores Nrf2 protein levels in HIT-T15 cells cultured in the presence of GS. (a)-(b) Semiquantitative RT-PCR of Nrf2 mRNA expression in HIT-T15 cells cultured 5 days in standard medium (CTR) or in medium containing AGEs (GS) in the presence or absence of 10 nmol/L GLP-1. (a) Representative agarose gel of three different experiments. (b) Quantification of densitometries of agarose gel bands. Data were represented as mean±SE of percentage change from CTR level (100%) ( ). (c)-(d) Western blot analysis of Nrf2 protein intracellular expression in HIT-T15 cells cultured in the same conditions as mRNA experiments. (c) Representative western blot of three different experiments. (d) Quantification of densitometries of western blot bands. Data were expressed as mean ± SE of fold induction relative to β-actin ( ). versus CTR; versus CTR; versus GS.
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