Sildenafil (Viagra) Protective Effects on Neuroinflammation: The Role of iNOS/NO System in an Inflammatory Demyelination Model

Figure 1

Western blotting for GFAP. (a) GFAP immunoblot of iNOS^−/− control (Cont), cuprizone (CPZ), and cuprizone plus sildenafil (CPZ + Sild) groups. (b) β-actin immunoblot. (c) Graph represents quantification and statistical analysis. The control group showed basal expression of GFAP. CPZ treatment induced a significant increase of this protein and sildenafil plus CPZ did not reduce GFAP expression, which remained higher in relation to the control group. The experiment was performed in triplicate (𝑛=5 animals/group). The results were expressed as mean ± SE. *𝑃<0.05, **𝑃<0.01 compared to control.

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