Chronic Pain Treatment: The Influence of Tricyclic Antidepressants on Serotonin Release and Uptake in Mast Cells

Figure 2

The effect of time of incubation on 5-HT uptake into mast cells. The mast cells were incubated with exogenous 5-HT (250 ng/sample) for 10, 30, or 60 min. (a) The effect of temperature of the medium on 5-HT uptake: mast cells were incubated with 5-HT at 37°C or at 0°C. (b) The effect of extracellular Ca²⁺ ions on 5-HT uptake: mast cells were incubated with 5-HT in the presence of extracellular Ca²⁺ ions (10⁻³mol/L) or in Ca²⁺-free medium. Each bar represents mean ± SEM of 5 experiments.

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(b)